cell culture 65 human oscc cell lines scc9  (ATCC)

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: (a) Molecular structure of the EDB-FN-specific contrast agent MT218. (b-d) Immunohistochemical staining of EDB-FN in human tissues with the EDB-FN-specific G4 antibody was (b) weak in normal tongue specimens (n=3), (c) strong in untreated primary OSCC specimens (n=7), (d) strong in OSCC metastatic specimens (n=2), and (e) moderate in neoadjuvant primary OSCC specimens (n=3). Scale bars in all panels: 200 μm.

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: (a) qRT-PCR mRNA analysis (n=3) and (b) western blot protein analysis (n=3) revealed differential and upregulated expression of EDB-FN in aggressive HSC3 and SCC4 cells. (c) Peptide binding study of spheroids with EDB-FN-specific ZD2-Cy5.5 (n=3) showed strong staining in HSC3 and SCC4 spheroids, but not CAL27 spheroids. Quantification of confocal images revealed (d) larger spheroids and (e) stronger ZD2-Cy5.5 staining in HSC3 and SCC4 spheroids in accordance with their EDB-FN expression levels. Data was analyzed with One-Way ANOVA and Fisher’s least significant difference post-hoc tests (*, p<0.05; **, p<0.01; ***, p<0.001; n.s., p>0.05). Scale bars for confocal images: 200 μm.

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Binding Assay, Staining

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: (a) Cells grown in 2D and 3D culture (n=3) showed high-risk features in HSC3 and SCC4 cells, but not CAL27 cells. (b) Transwell migration and invasion assays (n=3) demonstrated high invasive potential of HSC3 and SCC4 cells, but not CAL27 cells. Scale bars in all panels: 400 μm.

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: CNR analysis of contrast-enhanced tumors revealed (a) significant differential enhancement between CAL27, HSC3, and SCC4 tumors with MT218 in accordance with their EDB-FN expression, while (b) gadoteridol showed no difference in enhancement between the three models. Enhancement with MT218 was (c) generally lower than gadoteridol in CAL27 tumors, (d) generally higher than gadoteridol in HSC3 tumors, and (e) substantially higher than gadoteridol in SCC4 tumors. Maximum CNR enhancement in subjects with MT218 was (f) borderline lower than gadoteridol in CAL27 tumors (p=0.0957), (g) borderline higher than gadoteridol in HSC3 tumors (p=0.0639), and (h) significantly higher than gadoteridol in SCC4 tumors. (f-h) Open dots connected with a solid line represent matched mouse-by-mouse data, and solid dots connected with a dotted line represent cohort averages. (i) Average maximum CNR enhancement was significantly different between the three tumor models in accordance with their EDB-FN expression with MT218, but not with gadoteridol. Data was analyzed with Two-Way ANOVA and Fisher’s least significant difference post-hoc test (a-e,i) and unpaired T-tests (f-h) (*, p<0.05; **, p<0.01; ***, p<0.001; n.s., p>0.05).

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: (a) CAL27 tumors (n=5 for pre, 10, 20, and 30 minutes, n=4 for 40 minutes) showed slightly less enhancement with MT218 than gadoteridol. (b) HSC3 tumors (n=4) showed slightly more enhancement with MT218 than gadoteridol. (c) SCC4 tumors (n=4) showed substantially more enhancement with MT218 than gadoteridol. MT218 and gadoteridol were used at doses of 0.04 mmol/kg and 0.10 mmol/kg, respectively.

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Molecular imaging and biology

Article Title: Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer

doi: 10.1007/s11307-020-01524-6

Figure Lengend Snippet: (a) H&E staining of OSCC xenografts (n=3) showed various morphological features and stages of differentiation. (b) Immunohistochemistry for EDB-FN with the EDB-FN-specific G4 antibody (n=3) showed weak, moderate and strong staining in the epithelial regions of CAL27, HSC3, and SCC4 tumors, respectively, with little nonspecific staining. Scale bars: 600μm for H&E images, 200μm for immunohistochemistry images.

Article Snippet: Cell culture Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Immunohistochemistry

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Periostin expression in OSCC. (A) Representative images of periostin immunohistochemical staining (×200) and cells in the red boxes at ×400, and (B) mRNA expression of periostin examined by RT-qPCR in OSCC and healthy adjacent tissues. (C) Expression of periostin mRNA was examined by RT-qPCR in OSCC cells. (D) Protein expression of periostin was evaluated by western blotting in OSCC tissues and cell lines. *P<0.05 vs. corresponding control group. OSCC, oral squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: IOD of periostin in OSCC by immunohistochemical staining.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Association of the clinical characteristics of oral squamous cell carcinoma patients with positive (n=78; 86.7%) and negative (n=12; 13.3%) periostin expression.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Impact of periostin on the growth characteristics of OSCC cells. (A) mRNA and (B) protein levels of periostin were examined by reverse transcription- quantitative polymerase chain reaction and western blotting, respectively, after the cells were transfected with the overexpression plasmid or periostin siRNA. (C) Stimulatory effect of periostin on the proliferation of transfected SCC9 and SCC25 cells, as evaluated by the MTS assay. *P<0.05 vs. corresponding control group. OSCC, oral squamous cell carcinoma; siRNA, small interfering RNA; Ctrl, control.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Over Expression, Plasmid Preparation, MTS Assay, Control, Small Interfering RNA